Genetic Analysis in Bacillus pumilus by PBS1-Mediated Transduction
نویسندگان
چکیده
منابع مشابه
Linkage groups in Bacillus pumilus determined by bacteriophage PBS1-mediated transduction.
Two linkage groups were established in Bacillus pumilus NRRL B-3275 by bacteriophage PBS1 transduction. Group L contains the auxotrophic markers gly, his, and met. Group M contains the markers argO, met, ura, and cys.
متن کاملSome properties of the PBP1 transduction system in Bacillus pumilus.
Bacteriophage PBP1 is a flagella-specific virus that performs generalized transduction in strains of Bacillus pumilus. PBP1 is morphologically and serologically distinct from two other flagella-specific phages, PBS1 and SP-15, which perform generalized transduction in certain Bacillus species. The DNA extracted from PBP1 particles has a buoyant density of 1.690 g/cm(3) in cesium chloride gradie...
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Two strains of Bacillus pumilus (M11 and M38) and one strain each of Bacillus cereus (M27), Bacillus subtilis (M67), and Enterobacter agglomerans (M14) were identified from the air of Lancashire cotton mills. These strains were tested for cytopathic effects in Vero cells; B pumilus and B cereus strains were also examined for haemolytic activity, lecithinase production, and proteolytic action on...
متن کاملDivision mutants of Bacillus subtilis: isolation and PBS1 transduction of division-specific markers.
A procedure for the isolation of Bacillus subtilis mutants that appear to be defective in septum synthesis has been described. Fourteen mutants isolated with this technique were found to be located at four distinct loci on the B. subtilis chromosome. These have been designated divA, divB, divC, and divD. The four mutants in the divA group synthesize septa; however, they do so with a high freque...
متن کاملPlasmid deoxyribonucleic acid in Bacillus subtilis and Bacillus pumilus.
Two of eighteen strains of Bacillus subtilis examined contained covalently closed circular duplex deoxyribonucleic acid (DNA) of homogeneous size and buoyant density. Strain ATCC 15841 contained about 16 copies per chromosome of plasmid pPL1, a circular DNA element having a molecular weight of about 4.7 times 10(6) and a buoyant density of 1.700. Strain ATCC 7003 contained about one to two copi...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1970
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.101.2.603-608.1970